A Novel, Multiple-Antigen Pneumococcal Vaccine Protects against Lethal <i>Streptococcus pneumoniae</i> Challenge.

ABSTRACT: Current vaccination against Streptococcus pneumoniae uses vaccines based on capsular polysaccharides from selected serotypes and has led to nonvaccine serotype replacement disease. We have investigated an alternative serotype-independent approach, using multiple-antigen vaccines (MAV) prepared from S. pneumoniae TIGR4 lysates enriched for surface proteins by a chromatography step after culture under conditions that induce expression of heat shock proteins (Hsp; thought to be immune adjuvants). Proteomics and immunoblot analyses demonstrated that, compared to standard bacterial lysates, MAV was enriched with Hsps and contained several recognized protective protein antigens, including pneumococcal surface protein A (PspA) and pneumolysin (Ply). Vaccination of rodents with MAV i

SUBMITTER: Chan WY 

PROVIDER: S-EPMC6386546 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature