Drosophila melanogaster nonribosomal peptide synthetase Ebony encodes an atypical condensation domain.

ABSTRACT: The protein Ebony from Drosophila melanogaster plays a central role in the regulation of histamine and dopamine in various tissues through condensation of these amines with β-alanine. Ebony is a rare example of a nonribosomal peptide synthetase (NRPS) from a higher eukaryote and contains a C-terminal sequence that does not correspond to any previously characterized NRPS domain. We have structurally characterized this C-terminal domain and have discovered that it adopts the aryl-alkylamine-N-acetyl transferase (AANAT) fold, which is unprecedented in NRPS biology. Through analysis of ligand-bound structures, activity assays, and binding measurements, we have determined how this atypical condensation domain is able to provide selectivity for both the carrier protein-bound amino

SUBMITTER: Izore T 

PROVIDER: S-EPMC6386674 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature