Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism.

ABSTRACT: Although "genomically" humanized animals are invaluable tools for generating human disease models as well as for biomedical research, their development has been mainly restricted to mice via established transgenic-based and embryonic stem cell-based technologies. Since rats are widely used for studying human disease and for drug efficacy and toxicity testing, humanized rat models would be preferred over mice for several applications. However, the development of sophisticated humanized rat models has been hampered by the difficulty of complex genetic manipulations in rats. Additionally, several genes and gene clusters, which are megabase range in size, were difficult to introduce into rats with conventional technologies. As a proof of concept, we herein report the generation of genomically

SUBMITTER: Kazuki Y 

PROVIDER: S-EPMC6386724 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature