Colorimetric and Ratiometric Fluorescence Dual-Mode Sensing of Glucose Based on Carbon Quantum Dots and Potential UV/Fluorescence of o-Diaminobenzene.

ABSTRACT: A novel colorimetric and ratiometric fluorescence sensor was constructed by using carbon quantum dots (CQDs) and o-diaminobenzene (ODB). Unlike ODB by itself, ODB oxide (oxODB) not only emits fluorescence, but also produces ultraviolet (UV) absorption. Therefore, on the basis of the potential optical properties of ODB, glucose oxidase (Gox) and horseradish peroxidase (HRP) were introduced into a CQDs⁻ODB system for the quantitative oxidation of ODB. When glucose is present, it is oxidized by oxygen under the catalytic action of its oxidase to form hydrogen peroxide. Hydrogen peroxide is a strong oxidant that can rapidly oxidize ODB through the catalysis of horseradish peroxidase. oxODB can cause changes in the fluorescence ratio (I₅₅₀/I₄₄₆) and absorbance ratio (A/A₀)

SUBMITTER: Zhai H 

PROVIDER: S-EPMC6386939 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature