Analysis of Human Nuclear Protein Complexes by Quantitative Mass Spectrometry Profiling.

ABSTRACT: Analysis of protein complexes provides insights into how the ensemble of expressed proteome is organized into functional units. While there have been advances in techniques for proteome-wide profiling of cytoplasmic protein complexes, information about human nuclear protein complexes are very limited. To close this gap, we combined native size exclusion chromatography (SEC) with label-free quantitative MS profiling to characterize hundreds of nuclear protein complexes isolated from human glioblastoma multiforme T98G cells. We identified 1794 proteins that overlapped between two biological replicates of which 1244 proteins were characterized as existing within stably associated putative complexes. co-IP experiments confirmed the interaction of PARP1 with Ku70/Ku80 proteins and HDAC1 (histon

SUBMITTER: Connelly KE 

PROVIDER: S-EPMC6387628 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature