Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs.

ABSTRACT: Functionally and anatomically distinct cortical substructures, such as areas or layers, contain different principal neuron (PN) subtypes that generate output signals representing particular information. Various types of cortical inhibitory interneurons (INs) differentially but coordinately regulate PN activity. Despite a potential determinant for functional specialization of PN subtypes, the spatial organization of IN subtypes that innervate defined PN subtypes remains unknown. Here we develop a genetic strategy combining a recombinase-based intersectional labeling method and rabies viral monosynaptic tracing, which enables subtype-specific visualization of cortical IN ensembles sending inputs to defined PN subtypes. Our approach reveals not only cardinal but also underrepresented connecti

SUBMITTER: Yetman MJ 

PROVIDER: S-EPMC6387655 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature