Molecular cloning and characterization of six defensin genes from lentil plant (Lens culinaris L.).

ABSTRACT: Six full-length gene and cDNA sequences of defensin were identified from Lens culinaris L. plant. The identified genes and cDNAs were different in length and their coding sequences contained Knot1 functional domain. Phylogenetic analysis classified the identified defensins into two subfamilies. All defensin genes contained only one intron and had extracellular signal peptides. Secondary structures of identified defensins were completely composed of alpha helix and beta strand. Presence of conserved Cys amino acids and disulfide bridges, interaction with defense and signaling proteins and antimicrobial activity were other common features of these peptides. The identified defensins displayed differential expression pattern in the various tissues. The highest expression level of defens

SUBMITTER: Mir Drikvand R 

PROVIDER: S-EPMC6387662 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature