Selective profiling of ribosomes associated with yeast Upf proteins.

ABSTRACT: Ribosomes associated with nonsense-mediated decay factors Upf1, Upf2, or Upf3 were purified by immunoprecipitation, and enrichment and stoichiometry of Upf factors and ribosomal proteins were analyzed by western blot and mass spectrometry. Using a small RNA library preparation protocol that eliminates in-gel RNA and cDNA size selection and incorporates four random nucleotides on each side of the ribosome-protected RNA fragment allowed recovery, detection, and analysis of all size classes of protected fragments from a sample simultaneously.

SUBMITTER: Ganesan R 

PROVIDER: S-EPMC6387845 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature