In vitro Liver Zonation of Primary Rat Hepatocytes.

ABSTRACT: The ability of the liver to simultaneously carry out multiple functions is dependent on the metabolic heterogeneity of hepatocytes spatially located within a liver lobule spanning from the portal triad to the central vein. This complex zonal architecture of the liver, however, makes accurate in vitro modeling a challenge and often standard culture systems assume a homogenous model which may lead to inaccurate translatability of results. Here, we use a combination of mathematical modeling and experimental data to demonstrate a readily constructible in vitro flow system capable of liver zonation in primary rat hepatocytes. We show the differential expression of zonation markers, enhanced functionality when compared to standard static cultures and zone-specific metabolism and ce

SUBMITTER: Tomlinson L 

PROVIDER: S-EPMC6387900 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature