Human Mast Cells From Adipose Tissue Target and Induce Apoptosis of Breast Cancer Cells.

ABSTRACT: Mast cells (MC) are important immune sentinels found in most tissue and widely recognized for their role as mediators of Type I hypersensitivity. However, they also secrete anti-cancer mediators such as tumor necrosis factor alpha (TNF-α) and granulocyte-macrophage colony-stimulating factor (GM-CSF). The purpose of this study was to investigate adipose tissue as a new source of MC in quantities that could be used to study MC biology focusing on their ability to bind to and kill breast cancer cells. We tested several cell culture media previously demonstrated to induce MC differentiation. We report here the generation of functional human MC from adipose tissue. The adipose-derived mast cells (ADMC) are phenotypically and functionally similar to connective tissue expressing tryptase, chymase

SUBMITTER: Plotkin JD 

PROVIDER: S-EPMC6387946 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature