Effect of calcium ions and pH on the morphology and mechanical properties of hyaluronan brushes.

ABSTRACT: Hyaluronan (HA) is a linear, regular polysaccharide that plays as a chief structural and functional component in peri- and extracellular matrices, thus contributing significantly to many basic cellular processes. To understand more comprehensively the response of the supramolecular organization of HA polymers to changes in their aqueous environment, we study the effects of Ca2+ concentration and pH on the morphology and rigidity of films of end-grafted HA polymers on planar supports (HA brushes), as a well-defined in vitro model system of HA-rich matrices, by reflection interference contrast microscopy and quartz crystal microbalance. The thickness and softness of HA brushes decrease significantly with Ca2+ concentration but do not change with pH, within the physiological ranges of these p

SUBMITTER: Chen X 

PROVIDER: S-EPMC6388027 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature