Ablation of NTPDase2+ cells inhibits the formation of filiform papillae in tongue tip.

ABSTRACT:

Background

Lingual epithelia in the tongue tip are among the most rapidly regenerating tissues, but the mechanism of cell genesis in this tissue is still unknown. Previous study has suggested the existence of multiple stem cell pools in lingual epithelia and papillae. Like K14+ and Sox2+ cells, NTPDase2+ cells have characteristics of stem cells.

Methods

We employed a system using doxycycline to conditionally ablate NTPDase2+ cells in lingual epithelia and papillae by regulated expression of the diphtheria toxin A (DTA) gene. Transgenic lines, which expressed the rtTA gene in NTPDase2+ cells, were produced by pronuclear injection of zygotes from C57BL/6 mice using the BAC clone RP23-47P18. The NTPDase2-rtTA transgenic mice were crossed with the TetO-DTA transgenic animals. Th

SUBMITTER: Li F 

PROVIDER: S-EPMC6388074 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature