Continuous One Year Oral Administration of the Radiation Mitigator, MMS350, after Total-Body Irradiation, Restores Bone Marrow Stromal Cell Proliferative Capacity and Reduces Senescence in Fanconi Anemia (Fanca^-/-) Mice.

ABSTRACT: We quantitated age-related accumulation of senescent cells in irradiated Fanconi anemia (FA) (Fanca^-/- mouse cell lines in vitro, and monitored the effect of continuous administration (via drinking water) of the water-soluble radiation mitigator, MMS350, on tissues in vivo over one year after 7.5 Gy total-body irradiation (TBI). Irradiated Fanca^-/- mouse bone marrow stromal cell lines showed increased numbers of beta-galactosidase- and p21-positive senescent cells compared to Fanca^+/+ cell lines, which was reduced by MMS350. One week after 7.5 Gy TBI, Fanca^-/- mice showed increased numbers of senescent cells in spleen compared to Fanca^+/+ controls, decreased bone marrow cellularity, failure of explanted bone marrow to proliferate in vi

SUBMITTER: Sivananthan A 

PROVIDER: S-EPMC6388094 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature