A second HD mating type sublocus of Flammulina velutipes is at least di-allelic and active: new primers for identification of HD-a and HD-b subloci.

ABSTRACT:

Background

Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus has remained unclear.

Methods

To examine the function of the HD-a sublocus, genome sequences of a series of F. velutipes strains were analyzed and tested through series of amplification by specific primer sets. Furthermore, activity of di-allelic HD-a locus was confirmed by crossing strains with different combinations of HD-a and HD-b subloci.

Results

Sublocus HD-b contained a large variety of fixed Hd1/Hd2 gene pairs, while the HD-a subloc

SUBMITTER: Wang W 

PROVIDER: S-EPMC6388666 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature