Heterologous expression and antitumor activity analysis of syringolin from Pseudomonas syringae pv. syringae B728a.

ABSTRACT:

Background

Syringolin, synthesized by a mixed non-ribosomal peptide synthetase/polyketide synthetase in Pseudomonas syringae pv. syringae (Pss) B728a, is a novel eukaryotic proteasome inhibitor. Meanwhile, directly modifying large fragments in the PKS/NRPS gene cluster through traditional DNA engineering techniques is very difficult. In this study, we directly cloned the syl gene cluster from Pss B301D-R via Red/ET recombineering to effectively express syringolin in heterologous hosts.

Results

A 22 kb genomic fragment containing the sylA-sylE gene cluster was cloned into the pASK vector, and the obtained recombinant plasmid was transferred into Streptomyces coelicolor and Streptomyces lividans for the heterologous expression of syringolin. Transcriptional levels of recombina

SUBMITTER: Huang F 

PROVIDER: S-EPMC6389232 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature