Cross-site comparison of ribosomal depletion kits for Illumina RNAseq library construction.

ABSTRACT:

Background

Ribosomal RNA (rRNA) comprises at least 90% of total RNA extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment of mature poly-adenylated transcripts or targeted depletion of the rRNA fraction. The latter method is of particular interest because it is compatible with degraded samples such as those extracted from FFPE and also captures transcripts that are not poly-adenylated such as some non-coding RNAs. Here we provide a cross-site study that evaluates the performance of ribosomal RNA removal kits from Illumina, Takara/Clontech, Kapa Biosystems, Lexogen, New England Biolabs and Qiagen on intact and degraded RNA samples.

Results

We find that all of the

SUBMITTER: Herbert ZT 

PROVIDER: S-EPMC6389247 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature