Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids.

ABSTRACT: Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota. However, Paneth cell secretory responses remain debatable and the mechanisms that regulate the secretion are not well understood. Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota. Here, we report that microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen provides an

SUBMITTER: Yokoi Y 

PROVIDER: S-EPMC6389922 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature