Circadian regulation in human white adipose tissue revealed by transcriptome and metabolic network analysis.

ABSTRACT: Studying circadian rhythms in most human tissues is hampered by difficulty in collecting serial samples. Here we reveal circadian rhythms in the transcriptome and metabolic pathways of human white adipose tissue. Subcutaneous adipose tissue was taken from seven healthy males under highly controlled 'constant routine' conditions. Five biopsies per participant were taken at six-hourly intervals for microarray analysis and in silico integrative metabolic modelling. We identified 837 transcripts exhibiting circadian expression profiles (2% of 41619 transcript targeting probes on the array), with clear separation of transcripts peaking in the morning (258 probes) and evening (579 probes). There was only partial overlap of our rhythmic transcripts with published animal adipose and human blood tr

SUBMITTER: Christou S 

PROVIDER: S-EPMC6389935 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature