Small Peptide Ligands for Targeting EGFR in Triple Negative Breast Cancer Cells.

ABSTRACT: The efficacy of chemotherapy for cancer treatment can be increased by targeted drug delivery to the cancer cells. This is particularly important for triple negative breast cancer (TNBC) for which chemotherapy is a major form of treatment. Here we designed and screened a library of 30 peptides starting with a previously reported epidermal growth factor receptor (EGFR) targeting peptide GE11 (YHWYGYTPQNVI). A direct peptide array-whole cell binding assay, where the peptides are conjugated to a cellulose membrane, was used to identify four peptides with enhanced binding to TNBC cells. Next, the four peptides were synthesized as FITC-labelled soluble peptides to study their direct uptake by TNBC cells using flow cytometry. The results showed that peptide analogue 22 had several fold higher upt

SUBMITTER: Hossein-Nejad-Ariani H 

PROVIDER: S-EPMC6389950 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature