A simple high-throughput approach identifies actionable drug sensitivities in patient-derived tumor organoids.

ABSTRACT: Tumor organoids maintain cell-cell interactions, heterogeneity, microenvironment, and drug response of the sample they originate from. Thus, there is increasing interest in developing tumor organoid models for drug development and personalized medicine applications. Although organoids are in principle amenable to high-throughput screenings, progress has been hampered by technical constraints and extensive manipulations required by current methods. Here we introduce a miniaturized method that uses a simplified geometry by seeding cells around the rim of the wells (mini-rings). This allows high-throughput screenings in a format compatible with automation as shown using four patient-derived tumor organoids established from two ovarian and one peritoneal high-grade serous carcinomas and one ca

SUBMITTER: Phan N 

PROVIDER: S-EPMC6389967 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature