Long noncoding RNA GAS5 promotes apoptosis in primary nucleus pulposus cells derived from the human intervertebral disc via Bcl‑2 downregulation and caspase‑3 upregulation.

ABSTRACT: Nucleus pulposus cell (NPC) apoptosis serves an important role in intervertebral disc degeneration (IDD); however, the roles of long noncoding RNAs (lncRNAs) in this process remain unknown. The present study aimed to determine the effects of the lncRNA growth arrest‑specific transcript 5 (GAS5) on the apoptosis of primary human NPCs derived from the intervertebral disc, and to investigate the underlying mechanisms. TargetScan was used to predict the lncRNAs targeted by microRNA‑155 (miR‑155). Then, NPCs were subjected to lentivirus‑mediated transduction of miR‑155 or GAS5. A human lncRNA and mRNA array was used to screen differentially expressed lncRNAs following miR‑155 overexpression. GAS5 and miR‑155 expression levels were determined by reverse transcription‑quantitative polymerase chai

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6390029 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature