Rapid detection of colistin resistance protein MCR-1 by LC-MS/MS.

ABSTRACT:

Background

Colistin (polymyxin E) and polymixin B are important bactericidal antibiotics used in the treatment of serious infections caused by multi-drug resistant Gram-negative organisms. Transferrable plasmid-mediated colistin resistance, conferred by the product of the mcr-1 gene, has emerged as a global healthcare threat. Consequently, the rapid detection of the MCR-1 protein in clinical bacterial isolates has become increasingly important. We used a genoproteomic approach to identify unique peptides of the MCR-1 protein that could be detected rapidly by liquid chromatography tandem mass spectrometry (LC-MS/MS).

Methods

MCR-1 tryptic peptides that were efficiently ionized and readily detectable were characterized in a set of mcr-1-containing i

SUBMITTER: Wang H 

PROVIDER: S-EPMC6390366 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature