The Detergent Effect of Mesalazine Interferes with Phosphatidylcholine Binding to Mucin 2.

ABSTRACT:

Objectives

Therapeutically applied delayed-release phosphatidylcholine (PC) revealed mucosa protection and clinical improvement of ulcerative colitis. However, a recent trial with simultaneous application of delayed-release PC and mesalazine showed lack of efficacy. It is hypothesized that mesalazine acts as detergent to prohibit PC integration into mucus as target compartment, thus preventing topical mucus protection.

Methods

In vitro PC-binding studies with mucin 2 and intestinally differentiated CaCo2 cells as well as outcome analysis of a therapeutic trial with delayed-release PC and additional mesalazine.

Results

Choline-containing phospholipids, in particular PC, bind to mucin 2 as main scaffold protein of intestinal mucus to establish a hydrophobic barrier towa

SUBMITTER: Stremmel W 

PROVIDER: S-EPMC6390417 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature