Automated digital image quantification of histological staining for the analysis of the trilineage differentiation potential of mesenchymal stem cells.

ABSTRACT:

Background

Multipotent mesenchymal stem cells (MSCs) have the potential to repair and regenerate damaged tissues and are considered as attractive candidates for the development of cell-based regenerative therapies. Currently, there are more than 200 clinical trials involving the use of MSCs for a wide variety of indications. However, variations in their isolation, expansion, and particularly characterization have made the interpretation of study outcomes or the rigorous assessment of therapeutic efficacy difficult. An unbiased characterization of MSCs is of major importance and essential to guaranty that only the most suitable cells will be used. The development of standardized and reproducible assays to predict MSC potency is therefore mandatory. The currently used quantification

SUBMITTER: Eggerschwiler B 

PROVIDER: S-EPMC6390603 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature