Brain-wide distribution of reporter expression in five transgenic tetracycline-transactivator mouse lines.

ABSTRACT: The spatial pattern of transgene expression in tetracycline-controlled mouse models is governed primarily by the driver line used to introduce the tetracycline-controlled transactivator (tTA). Detailed maps showing where each tTA driver activates expression are therefore essential for designing and using tet-regulated models, particularly in brain research where cell type and regional specificity determine the circuits affected by conditional gene expression. We have compiled a comprehensive online repository of serial microscopic images showing brain-wide reporter expression for five commonly used tTA driver lines. We have spatially registered all images to a common three-dimensional mouse brain anatomical reference atlas for direct comparison of spatial distribution across lines. The hig

SUBMITTER: Lillehaug S 

PROVIDER: S-EPMC6390708 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature