Distribution of SiO₂ nanoparticles in 3D liver microtissues.

ABSTRACT:

Introduction

Nanoparticles (NPs) are used in numerous products in technical fields and biomedicine; their potential adverse effects have to be considered in order to achieve safe applications. Besides their distribution in tissues, organs, and cellular localization, their impact and penetration during the process of tissue formation occurring in vivo during liver regeneration are critical steps for establishment of safe nanomaterials.

Materials and methods

In this study, 3D cell culture of human hepatocarcinoma cells (HepG2) was used to generate cellular spheroids, serving as in vitro liver microtissues. In order to determine their differential distribution and penetration depth in HepG2 spheroids, SiO₂ NPs were applied either during or after spheroid formation. T

SUBMITTER: Fleddermann J 

PROVIDER: S-EPMC6390853 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature