The in vitro and in vivo anti-melanoma effects of hydroxyapatite nanoparticles: influences of material factors.

ABSTRACT:

Background

Treatment for melanoma is a challenging clinical problem, and some new strategies are worth exploring.

Purpose

The objective of this study was to investigate the in vitro and in vivo anti-melanoma effects of hydroxyapatite nanoparticles (HANPs) and discuss the involved material factors.

Materials and methods

Five types of HANPs, ie, HA-A, HA-B, HA-C, HA-D, and HA-E, were prepared by wet chemical method combining with polymer template and appropriate post-treatments. The in vitro effects of the as-prepared five HANPs on inhibiting the viability of A375 melanoma cells and inducing the apoptosis of the cells were evaluated by Cell Counting Kit-8 analysis, cell nucleus morphology observation, flow cytometer, and PCR analysis. The in vivo anti-melanoma effects o

SUBMITTER: Wu H 

PROVIDER: S-EPMC6391145 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature