Lipo-PEG-PEI complex as an intracellular transporter for protein therapeutics.

ABSTRACT:

Background

Protein or peptide drugs are emerging therapeutics for treating human diseases. However, current protein drugs are typically limited to acting on extracellular/cell membrane components associated with the diseases, while intracellular delivery of recombinant proteins replaces or replenishes faulty/missing proteins and remains inadequate. In this study, we developed a convenient and efficient intracellular protein delivery vehicle.

Materials and methods

A cationic liposomal polyethylenimine and polyethylene glycol complex (LPPC) was developed to noncovalently capture proteins for protein transfer into cells via endocytosis. β-glucuronidase (βG) was used in vitro and in vivo as a model enzyme to demonstrate the enzymatic activity of the intracellular transport of a

SUBMITTER: Lin YL 

PROVIDER: S-EPMC6391157 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature