Culture-independent analysis of hydrocarbonoclastic bacterial communities in environmental samples during oil-bioremediation.

ABSTRACT: To analyze microbial communities in environmental samples, this study combined Denaturing Gradient Gel Electrophoresis of amplified 16S rRNA-genes in total genomic DNA extracts from those samples with gene sequencing. The environmental samples studied were oily seawater and soil samples, that had been bioaugmented with natural materials rich in hydrocarbonoclastic bacteria. This molecular approach revealed much more diverse bacterial taxa than the culture-dependent method we had used in an earlier study for the analysis of the same samples. The study described the dynamics of bacterial communities during bioremediation. The main limitation associated with this molecular approach, namely of not distinguishing hydrocarbonoclastic taxa from others, was overcome by consulting the literature fo

SUBMITTER: Dashti N 

PROVIDER: S-EPMC6391274 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature