Metabolic adaptations during extreme anoxia in the turtle heart and their implications for ischemia-reperfusion injury.

ABSTRACT: ATP depletion and succinate accumulation during ischemia lead to oxidative damage to mammalian organs upon reperfusion. In contrast, freshwater turtles survive weeks of anoxia at low temperatures without suffering from oxidative damage upon reoxygenation, but the mechanisms are unclear. To determine how turtles survive prolonged anoxia, we measured ~80 metabolites in hearts from cold-acclimated (5 °C) turtles exposed to 9 days anoxia and compared the results with those for normoxic turtles (25 °C) and mouse hearts exposed to 30 min of ischemia. In turtles, ATP and ADP decreased to new steady-state levels during fasting and cold-acclimation and further with anoxia, but disappeared within 30 min of ischemia in mouse hearts. High NADH/NAD⁺ ratios were associated with succinate accu

SUBMITTER: Bundgaard A 

PROVIDER: S-EPMC6391391 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature