HMGA2 promotes long-term engraftment and myeloerythroid differentiation of human hematopoietic stem and progenitor cells.

ABSTRACT: Identification of determinants of fate choices in hematopoietic stem cells (HSCs) is essential to improve the clinical use of HSCs and to enhance our understanding of the biology of normal and malignant hematopoiesis. Here, we show that high-mobility group AT hook 2 (HMGA2), a nonhistone chromosomal-binding protein, is highly and preferentially expressed in HSCs and in the most immature progenitor cell subset of fetal, neonatal, and adult human hematopoiesis. Knockdown of HMGA2 by short hairpin RNA impaired the long-term hematopoietic reconstitution of cord blood (CB)-derived CB CD34⁺ cells. Conversely, overexpression of HMGA2 in CB CD34⁺ cells led to overall enhanced reconstitution in serial transplantation assays accompanied by a skewing toward the myeloerythroid li

SUBMITTER: Kumar P 

PROVIDER: S-EPMC6391669 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature