Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions.

ABSTRACT: Human enteroids-epithelial spheroids derived from primary gastrointestinal tissue-are a promising model to study pathogen-epithelial interactions. However, accessing the apical enteroid surface is challenging because it is enclosed within the spheroid. We developed a technique to reverse enteroid polarity such that the apical surface everts to face the media. Apical-out enteroids maintain proper polarity and barrier function, differentiate into the major intestinal epithelial cell (IEC) types, and exhibit polarized absorption of nutrients. We used this model to study host-pathogen interactions and identified distinct polarity-specific patterns of infection by invasive enteropathogens. Salmonella enterica serovar Typhimurium targets IEC apical surfaces for invasion via cytoskeletal rearrang

SUBMITTER: Co JY 

PROVIDER: S-EPMC6391775 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature