Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates.

ABSTRACT:

Background

Many microbes used for the rapid discovery and development of metabolic pathways have sensitivities to final products and process reagents. Isopentenol (3-methyl-3-buten-1-ol), a biogasoline candidate, has an established heterologous gene pathway but is toxic to several microbial hosts. Reagents used in the pretreatment of plant biomass, such as ionic liquids, also inhibit growth of many host strains. We explored the use of Corynebacterium glutamicum as an alternative host to address these constraints.

Results

We found C. glutamicum ATCC 13032 to be tolerant to both the final product, isopentenol, as well to three classes of ionic liquids. A heterologous mevalonate-based isopentenol pathway was engineered in C. glutamicum. Targeted proteomics

SUBMITTER: Sasaki Y 

PROVIDER: S-EPMC6391826 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature