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A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons.

ABSTRACT: Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and sub-threshold activity in neurons.

SUBMITTER: Sundukova M

PROVIDER: S-EPMC6391943 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons.

Sundukova Mayya M Prifti Efthymia E Bucci Annalisa A Kirillova Kseniia K Serrao Joana J Reymond Luc L Umebayashi Miwa M Hovius Ruud R Riezman Howard H Johnsson Kai K Heppenstall Paul A PA

Angewandte Chemie (International ed. in English) 20190125 8

Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and sub-threshold activity in neurons. ...[more]

PMID: 30569539

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