A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons.

ABSTRACT: Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and sub-threshold activity in neurons.

SUBMITTER: Sundukova M 

PROVIDER: S-EPMC6391943 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature