Impact of Preservation Method and 16S rRNA Hypervariable Region on Gut Microbiota Profiling.

ABSTRACT: Proper preservation of stool samples to minimize microbial community shifts and inactivate infectious agents is important for self-collected specimens requiring shipment to laboratories when cold chain transport is not feasible. In this study, we evaluated the performance of six preservation solutions (Norgen, OMNI, RNAlater, CURNA, HEMA, and Shield) for these aspects. Following storage of human stool samples with these preservatives at room temperature for 7 days, three hypervariable regions of the bacterial 16S rRNA gene (V1-V2, V3-V4, and V4) were amplicon sequenced. We found that samples collected in two preservatives, Norgen and OMNI, showed the least shift in community composition relative to -80°C standards compared with other storage conditions, and both efficiently inhibited the g

SUBMITTER: Chen Z 

PROVIDER: S-EPMC6392095 | biostudies-literature | 2019 Jan-Feb

REPOSITORIES: biostudies-literature