Keratin 8 is a potential self-antigen in the coronary artery disease immunopeptidome: A translational approach.

ABSTRACT:

Background

Inflammation is an important risk factor in atherosclerosis, the underlying cause of coronary artery disease (CAD). Unresolved inflammation may result in maladaptive immune responses and lead to immune reactivity to self-antigens. We hypothesized that inflammation in CAD patients would manifest in immune reactivity to self-antigens detectable in soluble HLA-I/peptide complexes in the plasma.

Methods

Soluble HLA-I/peptide complexes were immuno-precipitated from plasma of male acute coronary syndrome (ACS) patients or age-matched controls and eluted peptides were subjected to mass spectrometry to generate the immunopeptidome. Self-peptides were ranked according to frequency and signal intensity, then mouse homologs of selected peptides were used to test immunologic

SUBMITTER: Mihailovic PM 

PROVIDER: S-EPMC6392305 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature