Sumoylation of DNA-bound transcription factor Sko1 prevents its association with nontarget promoters.

ABSTRACT: Sequence-specific transcription factors (TFs) represent one of the largest groups of proteins that is targeted for SUMO post-translational modification, in both yeast and humans. SUMO modification can have diverse effects, but recent studies showed that sumoylation reduces the interaction of multiple TFs with DNA in living cells. Whether this relates to a general role for sumoylation in TF binding site selection, however, has not been fully explored because few genome-wide studies aimed at studying such a role have been reported. To address this, we used genome-wide analysis to examine how sumoylation regulates Sko1, a yeast bZIP TF with hundreds of known binding sites. We find that Sko1 is sumoylated at Lys 567 and, although many of its targets are osmoresponse genes, the level of Sko1 su

SUBMITTER: Sri Theivakadadcham VS 

PROVIDER: S-EPMC6392331 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature