Cell Therapy for Cystic Fibrosis Lung Disease: Regenerative Basal Cell Amplification.

ABSTRACT: The human airway epithelium is regenerated by basal cells. Thus, basal cell therapy has the potential to cure cystic fibrosis (CF) lung disease. We previously reported that the human basal cells repopulated the mouse airway epithelium after transplantation, and we estimated that 60 million cells would be needed to treat a human patient. To further develop cell therapy, we compared the proliferation potential of non-CF and CF tissue-derived bronchial basal cells. Three methods were used: regenerative cell frequency, burst size, and cell division frequency. Second, we used a serial passage strategy to determine if CF basal cells could be amplified to the estimated therapeutic dose. These studies evaluated that tissue-derived bronchial basal cells and the basal cells that were recovered by br

SUBMITTER: Hayes D 

PROVIDER: S-EPMC6392379 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature