A genetically encoded near-infrared fluorescent calcium ion indicator.

ABSTRACT: We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca²⁺) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca²⁺ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca²⁺ imaging in combination with other optogenetic indicators and actuators.

SUBMITTER: Qian Y 

PROVIDER: S-EPMC6393164 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature