Discordance between histologic and visual assessment of tissue viability in excised burn wound tissue.

ABSTRACT: The regenerative capacity of burn wounds, and the need for surgical intervention, depends on wound depth. Clinical visual assessment is considered the gold standard for burn depth assessment but it remains a subjective and inaccurate method for tissue evaluation. The purpose of this study was to compare visual assessment with microscopic and molecular techniques for human burn depth determination, and illustrate differences in the evaluation of tissue for potential regenerative capacity. Using intraoperative visual assessment, patients were identified as having deep partial thickness or full thickness burn wounds. Tangential excisions of burn tissue were processed with hematoxylin and eosin to visualize tissue morphology, lactate dehydrogenase assay to ascertain cellular viability, and Ker

SUBMITTER: Karim AS 

PROVIDER: S-EPMC6393178 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature