Genetic and Phenotypic Features to Screen for Putative Adherent-Invasive Escherichia coli.

ABSTRACT: To date no molecular tools are available to identify the adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease and colonizes the intestine of different hosts. Current techniques based on phenotypic screening of isolates are extremely time-consuming. The aim of this work was to search for signature traits to assist in rapid AIEC identification. The occurrence of at least 54 virulence genes (VGs), the resistance to 30 antibiotics and the distribution of FimH and ChiA amino acid substitutions was studied in a collection of 48 AIEC and 56 non-AIEC isolated from the intestine of humans and animals. χ² test was used to find frequency differences according to origin of isolation, AIEC phenotype and phylogroup. Mann-Whitney test was a

SUBMITTER: Camprubi-Font C 

PROVIDER: S-EPMC6393329 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature