A novel mutation tolerant padlock probe design for multiplexed detection of hypervariable RNA viruses.

ABSTRACT: The establishment of a robust detection platform for RNA viruses still remains a challenge in molecular diagnostics due to their high mutation rates. Newcastle disease virus (NDV) is one such RNA avian virus with a hypervariable genome and multiple genotypes. Classical approaches like virus isolation, serology, immunoassays and RT-PCR are cumbersome, and limited in terms of specificity and sensitivity. Padlock probes (PLPs) are known for allowing the detection of multiple nucleic acid targets with high specificity, and in combination with Rolling circle amplification (RCA) have permitted the development of versatile pathogen detection assays. In this work, we aimed to detect hypervariable viruses by developing a novel PLP design strategy capable of tolerating mutations while preserving hig

SUBMITTER: Ciftci S 

PROVIDER: S-EPMC6393471 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature