Multiple mutations in the nicotinic acetylcholine receptor Ccα6 gene associated with resistance to spinosad in medfly.

ABSTRACT: Spinosad is an insecticide widely used for the control of insect pest species, including Mediterranean fruit fly, Ceratitis capitata. Its target site is the α6 subunit of the nicotinic acetylcholine receptors, and different mutations in this subunit confer resistance to spinosad in diverse insect species. The insect α6 gene contains 12 exons, with mutually exclusive versions of exons 3 (3a, 3b) and 8 (8a, 8b, 8c). We report here the selection of a medfly strain highly resistant to spinosad, JW-100 s, and we identify three recessive Ccα6 mutant alleles in the JW-100 s population: (i) Ccα6^3aQ68* containing a point mutation that generates a premature stop codon on exon 3a (3aQ68*); (ii) Ccα6^3aAG>AT containing a point mutation in the 5' splicing site of exon 3a (3aAG > AT

SUBMITTER: Urena E 

PROVIDER: S-EPMC6393475 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature