Forskolin and IBMX Induce Neural Transdifferentiation of MSCs Through Downregulation of the NRSF.

ABSTRACT: Neural differentiation of mesenchymal stem cells is a controversial phenomenon, as it would require transdifferentiation across the mesoderm-ectoderm barrier. However, several laboratories have observed that MSCs are able to be induced to express neural characteristics. Previously, we demonstrated that the cAMP-elevating agents, forskolin and IBMX, induced neural-like differentiation of MSCs, including expression of neural markers and increased sensitivity to neurotransmitters. However, due to the broad range of effects that forskolin and IBMX can elicit through the intracellular second messenger, cAMP, a better mechanistic understanding is required. Here, we show that neural induction by forskolin and IBMX is dependent on downregulation of expression of the master transcriptional regulato

SUBMITTER: Thompson R 

PROVIDER: S-EPMC6393535 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature