2C-ChIP: measuring chromatin immunoprecipitation signal from defined genomic regions with deep sequencing.

ABSTRACT:

Background

Understanding how transcription occurs requires the integration of genome-wide and locus-specific information gleaned from robust technologies. Chromatin immunoprecipitation (ChIP) is a staple in gene expression studies, and while genome-wide methods are available, high-throughput approaches to analyze defined regions are lacking.

Results

Here, we present carbon copy-ChIP (2C-ChIP), a versatile, inexpensive, and high-throughput technique to quantitatively measure the abundance of DNA sequences in ChIP samples. This method combines ChIP with ligation-mediated amplification (LMA) and deep sequencing to probe large genomic regions of interest. 2C-ChIP recapitulates results from benchmark ChIP approaches. We applied 2C-ChIP to the HOXA cluster to find that a region wh

SUBMITTER: Wang XQD 

PROVIDER: S-EPMC6394006 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature