Polar localization of the ATPase ClpV-5 occurs independent of type VI secretion system apparatus proteins in Burkholderia thailandensis.

ABSTRACT:

Objective

ClpV, the ATPase of the type VI secretion system (T6SS) recycles cytoplasmic T6SS proteins following effector translocation. Fluorescent protein fusions to ClpV showed that it localizes to discrete and dynamic foci. ClpV-1-sfGFP of the bacterial cell targeting T6SS-1 of Burkholderia thailandensis exhibits a virtually random localization, whereas ClpV-5-sfGFP of the T6SS-5 targeting host cells is located at one or both poles. The mechanisms underlying the differential localization pattern are not known. Previous analysis of T6SSs, which target bacterial cells revealed that ClpV foci formation is dependent on components of the T6SS. Here, we investigated if the T6SS-5 apparatus confers polar localization of ClpV-5.

Results

ClpV-5-sfGFP foci formation and localization

SUBMITTER: Lennings J 

PROVIDER: S-EPMC6394029 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature