Comparison of efficiency and time to regeneration of Agrobacterium-mediated transformation methods in Medicago truncatula.

ABSTRACT:

Background

Tissue culture transformation of plants has an element of art to it, with protocols passed on between labs but often not directly compared. As Medicago truncatula has become popular as a model system for legumes, rapid transformation is critical, and many protocols exist, with varying results.

Results

The M. truncatula ecotypes, R108 and A17, were utilized to compare the effect of a modification to a previously used protocol based on shoot explants on the percentage of transformed plants produced from calli. This percentage was then compared to that of two additional transformation protocols based on root explants in the R108 ecotype. Variations in embryonic tissue sources, media components, time for transformation, and vectors were analyzed.

Conc

SUBMITTER: Wen L 

PROVIDER: S-EPMC6394069 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature