Assessment and site-specific manipulation of DNA (hydroxy-)methylation during mouse corticogenesis.

ABSTRACT: Dynamic changes in DNA (hydroxy-)methylation are fundamental for stem cell differentiation. However, the signature of these epigenetic marks in specific cell types during corticogenesis is unknown. Moreover, site-specific manipulation of cytosine modifications is needed to reveal the significance and function of these changes. Here, we report the first assessment of (hydroxy-)methylation in neural stem cells, neurogenic progenitors, and newborn neurons during mammalian corticogenesis. We found that gain in hydroxymethylation and loss in methylation occur sequentially at specific cellular transitions during neurogenic commitment. We also found that these changes predominantly occur within enhancers of neurogenic genes up-regulated during neurogenesis and target of pioneer transcription fact

SUBMITTER: Noack F 

PROVIDER: S-EPMC6394126 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature