Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis.

ABSTRACT: INTRODUCTION:Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is a powerful approach for visualizing the localization of metabolites. OBJECTIVES:A method to keep the shape of plant tissue needs to be developed for MALDI-IMS. METHODS:The method was developed using transfer tape and double-sided conductive tape. RESULTS:MALDI-IMS analysis using the developed method enabled to perform segmentation and correlation analysis of mass features. CONCLUSION:This proof-of-concept study showed that rutin localizes in the epidermis, developing tissue, and protoxylem in Asparagus officinalis.

SUBMITTER: Nakabayashi R 

PROVIDER: S-EPMC6394462 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature