Molecular Cloning and Expression Analysis of 5-hydroxytryptamine Receptor 7 in Ant Polyrhachis vicina Roger (Hymenoptera: Formicidae).

ABSTRACT: Serotonin (5-hydroxytryptamine [5-HT]) is a monoamine neurotransmitter that plays an important role in regulating a variety of physiological and behavioral activities. In this study, the 5-HT7 receptor gene was cloned from the ant Polyrhachis vicina Roger (1863). The complete Pv5-HT7 receptor cDNA is 3054 bp, including a 5'-untranslated region (UTR) of 790 bp, a 3'-UTR of 752 bp and an open reading frame of 1512 bp encoding polypeptide of 503 amino acids. Hydrophobic analysis suggests that seven trans-membrane domains are the major sequence characteristic of the Pv5-HT7 receptor. In addition, the Pv5-HT7 receptor has three potential N-glycosylation sites, a palmitoylation site, three protein kinase A phosphorylation sites, and four protein kinase C phosphorylation sites. Phylogenetic analy

SUBMITTER: Yang S 

PROVIDER: S-EPMC6394970 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature